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It was given (i) intraperitoneally or (ii) intragastrically or (iii) topically at the swollen brain through an additional midline burr hole placed approximately 2 mm rostral to the horizontal line connecting the two parasagittal burr holes, making a triangle with them and overlying the sagittal sinus in the dural

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Aldosterone Effects • Acts on distal tubules and collecting ducts in the kidney • Increases sodium and water reabsorption • Promotes potassium excretion • Net effect: increases blood volume and blood pressure ✅ Feedback Regulation • High blood pressure / high sodium → decreases renin release • Low blood pressure / low sodium → increases renin release ✅ Clinical Significance • Hypertension → overactive RAAS contributes to high BP • Heart failure → compensatory RAAS activation can worsen fluid overload • Kidney disease → RAAS blockers protect renal function • Drugs targeting RAAS: • ACE inhibitors (e.g., enalapril, lisinopril) • Angiotensin II receptor blockers (ARBs) (e.g., losartan) • Direct renin inhibitors (e.g., aliskiren) • Aldosterone antagonists (e.g., spironolactone, eplerenone) ✅ Summary 1