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In further research, it was found that the neuroprotection mediated by berberine after ischemia involves the AKT/glycogen synthase kinase-3β (GSK3β)/extracellular signal-regulated kinase 1/2 (ERK 1/2) signal pathway, and inhibits Jun amino-terminal kinases (JNK) and caspase-3 activity ( Berberine inhibits neuronal apoptosis by regulating autophagy-related proteins [Microtubule-associated protein 1A/1B-light chain 3 (LC3), Beclin-1, p62], and apoptosis regulatory proteins [caspase 3, caspase 8, caspase 9, poly ADP-ribose polymerase (PARP), and B-cell lymphoma 2 (Bcl-2)/Bcl-2 associated X (Bax)] ( In addition, animal experiments and cell experiments have confirmed that berberine reduces the levels of extracellular and intracellular Aβ, increases the levels of LC3-II, Beclin-1, hVps34 and cathepsin-D, and reduced the levels of P62, Bcl-2, amyloid precursor protein (APP), and beta-secretase 1 (BACE1)