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Briefly, a reagent solution containing 0.56 mM phenol red (Alfa Aesar, UK) and 17 U/ml horseradish peroxidase (Type II, Sigma-Aldrich, Israel) in 10 mM glycyl-glycine buffer, pH 7.5, 50 mM NaCl, was added at a 1:1 volumetric ratio to the analyzed sample, and the mixture was incubated at room temperature for 2 min (5 min produced similar results), followed by the addition of NaOH to the final concentration of 50 mM to reach pH 12.5