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Overall, current data suggest that probiotic bacteria can contribute to attenuation of HPA axis hyperreactivity and improve stress resilience, but they should be considered adjunctive modulators rather than primary regulators

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Briefly, the animals were synchronized by hypochlorite bleaching, hatched overnight in M9 (3 g/l KH2PO4, 6 g/l Na2HPO4, 5 g/l NaCl, 1 µM MgSO4) buffer, and subsequently cultured at 20 °C on nematode growth medium (NGM) (CaCl2 1mM, MgSO4 1 mM, cholesterol 5 µg/ml, 250 µM KH2PO4 pH 6, Agar 17 g/l, NaCl 3g/l, casein 7.5g/l) plates seeded with the E