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CYP1A2, 2D6, and 3A4 have also been shown to activate APAP in various model systems (80–82)
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The human renal proximal tubular cell line (HK-2 cell line) was obtained from the American Type Culture Collection (ATCC®, CRL-2190) and cultured in Dulbecco's modified Eagle's medium (DMEM)/F-12 (Thermo Fisher Scientific, 11330057, New York, USA) supplemented with 1% penicillin‒streptomycin (Thermo Fisher Scientific, 10378016) and 10% fetal bovine serum (Thermo Fisher Scientific, 10099158)
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