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Transforming Growth Factor-β-mediated attenuation of cardio-renal oxidative stress, inflammation and fibrosis by L-arginine in fludrocortisone acetate induced-hypertensive rats - ScienceDirect

The homogenates were centrifuged at 16,000 g for 10 min, the supernatants were collected, and remaining pellets were washed with 100 µL of ice-cold methanol with 0.1% acetic acid and 0.1% BHT and centrifuged at 16,000 g for 10 min

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Sander, I