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Key Research Areas for FOXO4-DRI FOXO4–p53 protein-protein interaction disruption assays — co-immunoprecipitation (CO-IP) and competitive binding studies Senescent cell identification and selective elimination assays — SA-β-galactosidase, p16, p21, p53 staining combined with FOXO4-DRI apoptosis induction p53 TAD2 binding, nuclear exclusion, and mitochondrial translocation studies — phospho-p53 (Ser15) pathway research BAX/BCL-2/Caspase-3 intrinsic apoptosis pathway investigations in senescent cell models SASP (Senescence-Associated Secretory Phenotype) characterisation and suppression — IL-6, IL-8, MMP, VEGF, CXCL2/3 pathway studies Tissue-specific senescent cell biology — fibroblasts, endothelial cells, Leydig cells, chondrocytes, epithelial cells Vascular ageing pathway research — endothelial cell senescence, aortic function, and ROS/oxidative stress studies Senescent Leydig cell and male reproductive ageing models — FOXO4 nuclear translocation and testosterone synthesis pathway research Chondrocyte senescence and cartilage biology — senescent cell removal and chondrogenic potential restoration studies Keloid and pathological scar biology — senescent fibroblast elimination and SASP suppression in fibrotic tissue models Senescent cancer cell elimination — FOXO4-DRI as a senolytic tool in oncology research models Comparative senolytic research — FOXO4-DRI vs

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Jia, 2007