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Even though there are different peptide-protein docking platforms currently available, peptides have a greater challenge as ligands than small chemical compounds, due to their size, conformational flexibility, and lateral group complexity (Scharbert and Strodel, 2025)
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The ex vivo synthesized sequences of FAPCAR were cloned into IVT template plasmid as the ORF region which also carried T7 promoter, modified 5’ UTR, kozak sequence, 3’ UTR, and a 110 nt-long poly (A) tail 59,60,61
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