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The primers used in the PCR were as follows: For MTHFR rs1801133 variant (C/T): Forward primer: 5′-CCTCTCCTGACTGTCATCCC–3′ Reverse primer: 5′-GCCTTCACAAAGCGGAAGAA–3′ For MTHFR rs1801131 variant (A/C): Forward primer: 5′-TACCTGAAGAGCAAGTCCCC–3′ Reverse primer: 5′-ACAGGATGGGGAAGTCACAG–3′ Sequencing Analysis The Sanger Sequencing technique was used for DNA sequence analysis, along with the Big Dye Terminator Cycle Sequencing Kit (Applied Biosystems Inc., Foster City, CA, USA)