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Supernatants (900 μl) were transferred to brown glass vials for further analysis using an Accela LC system controlled by Aria software version 1.6.3 and coupled to a high resolution Q-Exactive system equipped with a heated electrospray ionization (HESI)-II source and controlled by Xcalibur 2.2 SP1.48 software (all of ThermoFisher, Dreieich, Germany)
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