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The bacterial suspension was serially diluted after being resuspended in sterile PBS, and 100 μl bacterial solution was taken into 900 μl BHI broth containing 10 mM H 2 O 2 , 10 mM FeCl 3 , and 5 mM CuSO 4 for 1 h, respectively

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They’re produced naturally when your body burns oxygen for energy (in your mitochondria), but also from: Pollution and cigarette smoke Alcohol and processed food UV light and stress In small amounts, free radicals are fine - they’re actually part of your immune defence