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Upon stimulation, compression is removed, and the muscle relaxes

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Biochemical and enzyme analysis The freeze-dried leaf sample (1 g) was thoroughly macerated with 10 mL of a cold enzyme extraction solution containing 0.2% polyvinylpolypyrrolidone (PVP), 0.1 mM EDTA, and 50 mM potassium phosphate buffer (pH 7.8)