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GST expression vectors like the pGEX (Hakes and Dixon, 1992) or pCold-GST (Hayashi and Kojima, 2008) usually contain a protease recognition site between the fusion tag coding gene and the target protein coding gene for GST tag’s removal after or during protein purification

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Other options include: Topical skincare using ingredients like niacinamide or azelaic acid, which often take longer to produce visible changes Laser treatments , which target deeper pigmentation but may involve downtime Chemical peels , suitable for surface pigmentation but not ideal for all skin types Injectable antioxidants and polynucleotide skin boosters appeal to those seeking targeted, gradual results without surgery